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Format
inperson
Location and Room
Building 2, Room 449
Address
182 MEMORIAL DR, Cambridge, MA 02139
Speaker: Surabhi K Sreenivas (Harvard)
Title: Breaking the Spectral Barrier: Novel Flux Enabled Multiplexing and FLIM in Single Molecule Localization Microscopy
Abstract:
Single Molecule Localization Microscopy (SMLM) offers nanometer scale resolution, however true multiplexed imaging remains limited by the need for multiple lasers, spectral separation, and complex detection hardware. This talk introduces a new class of flux-based strategies that overcome these limitations and enable both hardware minimal multiplexing and low-cost fluorescence lifetime imaging (FLIM).
A novel demixing approach is presented based on fluorescence flux, an intrinsic property of fluorophores that, when illumination and detection conditions are controlled, provides a species-specific signature independent of emission spectra. This enables simultaneous...
Format
inperson
Location and Room
Building 2, Room 449
Address
182 MEMORIAL DR, Cambridge, MA 02139
Speaker: Surabhi K Sreenivas (Harvard)
Title: Breaking the Spectral Barrier: Novel Flux Enabled Multiplexing and FLIM in Single Molecule Localization Microscopy
Abstract:
Single Molecule Localization Microscopy (SMLM) offers nanometer scale resolution, however true multiplexed imaging remains limited by the need for multiple lasers, spectral separation, and complex detection hardware. This talk introduces a new class of flux-based strategies that overcome these limitations and enable both hardware minimal multiplexing and low-cost fluorescence lifetime imaging (FLIM).
A novel demixing approach is presented based on fluorescence flux, an intrinsic property of fluorophores that, when illumination and detection conditions are controlled, provides a species-specific signature independent of emission spectra. This enables simultaneous...